dapi channel brightline dapi-5060c-nte-zero Search Results


brightline filters af488  (IDEX)
90
IDEX brightline filters af488
a Diagram of the Xist/Tsix locus showing Xist exons (green), Tsix exons (magenta), introns (lines), and probe positions for Xist introns (green) and Tsix 3’ introns (magenta). Polymerase positions suggest potential co-transcription of Xist and Tsix. b Conceptual diagram showing expected outcomes with or without TI. If TI occurs, Xist (green) and Tsix 3’ (magenta) signals won’t colocalize (left). Without TI, colocalization occurs (right). c Example image showing <t>DAPI</t> (blue), Xist (green), Tsix 3’ (magenta), and colocalization (white). d-f Similar diagrams and images as (A-C), but for Tsix 5’. With TI, more Xist and Tsix 5’ colocalization occurs than with Tsix 3’ (left); without TI, colocalization remains consistent (right). g-h Joint probability distributions of nascent Tsix (x-axis) and Xist (y-axis) transcripts. Real data is on the left, randomized data on the right. Rho and p values indicate Spearman correlations (real data) and 2D KS test results (randomized data). Analysis includes 625 cells for Tsix 5’ and 651 cells for Tsix 3’. i-n Quantification of colocalization at transcription sites, varying by nascent transcription levels (low, medium, high). Colocalization is plotted by search radius (x-axis, cubic scale) and average events per transcription site (y-axis). Solid line is the mean and shaded area is the standard deviation. Dashed lines is the background fit. Dotted line is the colocalization by chance. i, k, m Quantification of colocalization of Xist with Tsix 3’ depending on the nascent transcription of Xist. Low-level nascent Xist (i), shows colocalization with Tsix 3’ (18 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 3’ (54 tsites) (k), and a lower fraction of high-level Xist colocalizes with Tsix 3’ (11 tsites) (m). A total of n = 651 cells are quantified in this analysis. j, l, n Quantification of colocalization of Xist with Tsix 5’ depending on the nascent transcription of Xist. Low-level nascent Xist (j), shows colocalization with Tsix 5’ (30 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 5’ (68 tsites) (l), and similarly high fraction of high-level Xist colocalizes with Tsix 5’ (33 tsites) (n). A total of n = 625 cells are quantified in this analysis.
Brightline Filters Af488, supplied by IDEX, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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brightline filters af488 - by Bioz Stars, 2026-04
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brightline single-band filter sets (dapi-5060c nte-zero, fitc-3540c nte-zero and txred-4040c nte-zero)  (IDEX)
90
IDEX brightline single-band filter sets (dapi-5060c nte-zero, fitc-3540c nte-zero and txred-4040c nte-zero)
a Diagram of the Xist/Tsix locus showing Xist exons (green), Tsix exons (magenta), introns (lines), and probe positions for Xist introns (green) and Tsix 3’ introns (magenta). Polymerase positions suggest potential co-transcription of Xist and Tsix. b Conceptual diagram showing expected outcomes with or without TI. If TI occurs, Xist (green) and Tsix 3’ (magenta) signals won’t colocalize (left). Without TI, colocalization occurs (right). c Example image showing <t>DAPI</t> (blue), Xist (green), Tsix 3’ (magenta), and colocalization (white). d-f Similar diagrams and images as (A-C), but for Tsix 5’. With TI, more Xist and Tsix 5’ colocalization occurs than with Tsix 3’ (left); without TI, colocalization remains consistent (right). g-h Joint probability distributions of nascent Tsix (x-axis) and Xist (y-axis) transcripts. Real data is on the left, randomized data on the right. Rho and p values indicate Spearman correlations (real data) and 2D KS test results (randomized data). Analysis includes 625 cells for Tsix 5’ and 651 cells for Tsix 3’. i-n Quantification of colocalization at transcription sites, varying by nascent transcription levels (low, medium, high). Colocalization is plotted by search radius (x-axis, cubic scale) and average events per transcription site (y-axis). Solid line is the mean and shaded area is the standard deviation. Dashed lines is the background fit. Dotted line is the colocalization by chance. i, k, m Quantification of colocalization of Xist with Tsix 3’ depending on the nascent transcription of Xist. Low-level nascent Xist (i), shows colocalization with Tsix 3’ (18 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 3’ (54 tsites) (k), and a lower fraction of high-level Xist colocalizes with Tsix 3’ (11 tsites) (m). A total of n = 651 cells are quantified in this analysis. j, l, n Quantification of colocalization of Xist with Tsix 5’ depending on the nascent transcription of Xist. Low-level nascent Xist (j), shows colocalization with Tsix 5’ (30 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 5’ (68 tsites) (l), and similarly high fraction of high-level Xist colocalizes with Tsix 5’ (33 tsites) (n). A total of n = 625 cells are quantified in this analysis.
Brightline Single Band Filter Sets (Dapi 5060c Nte Zero, Fitc 3540c Nte Zero And Txred 4040c Nte Zero), supplied by IDEX, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/brightline single-band filter sets (dapi-5060c nte-zero, fitc-3540c nte-zero and txred-4040c nte-zero)/product/IDEX
Average 90 stars, based on 1 article reviews
brightline single-band filter sets (dapi-5060c nte-zero, fitc-3540c nte-zero and txred-4040c nte-zero) - by Bioz Stars, 2026-04
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brightline single-band filter sets 166 (dapi-5060c-nte-zero, fitc-3540c-nte-zero and txred-4040c-nte-zero)  (IDEX)
90
IDEX brightline single-band filter sets 166 (dapi-5060c-nte-zero, fitc-3540c-nte-zero and txred-4040c-nte-zero)
a Diagram of the Xist/Tsix locus showing Xist exons (green), Tsix exons (magenta), introns (lines), and probe positions for Xist introns (green) and Tsix 3’ introns (magenta). Polymerase positions suggest potential co-transcription of Xist and Tsix. b Conceptual diagram showing expected outcomes with or without TI. If TI occurs, Xist (green) and Tsix 3’ (magenta) signals won’t colocalize (left). Without TI, colocalization occurs (right). c Example image showing <t>DAPI</t> (blue), Xist (green), Tsix 3’ (magenta), and colocalization (white). d-f Similar diagrams and images as (A-C), but for Tsix 5’. With TI, more Xist and Tsix 5’ colocalization occurs than with Tsix 3’ (left); without TI, colocalization remains consistent (right). g-h Joint probability distributions of nascent Tsix (x-axis) and Xist (y-axis) transcripts. Real data is on the left, randomized data on the right. Rho and p values indicate Spearman correlations (real data) and 2D KS test results (randomized data). Analysis includes 625 cells for Tsix 5’ and 651 cells for Tsix 3’. i-n Quantification of colocalization at transcription sites, varying by nascent transcription levels (low, medium, high). Colocalization is plotted by search radius (x-axis, cubic scale) and average events per transcription site (y-axis). Solid line is the mean and shaded area is the standard deviation. Dashed lines is the background fit. Dotted line is the colocalization by chance. i, k, m Quantification of colocalization of Xist with Tsix 3’ depending on the nascent transcription of Xist. Low-level nascent Xist (i), shows colocalization with Tsix 3’ (18 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 3’ (54 tsites) (k), and a lower fraction of high-level Xist colocalizes with Tsix 3’ (11 tsites) (m). A total of n = 651 cells are quantified in this analysis. j, l, n Quantification of colocalization of Xist with Tsix 5’ depending on the nascent transcription of Xist. Low-level nascent Xist (j), shows colocalization with Tsix 5’ (30 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 5’ (68 tsites) (l), and similarly high fraction of high-level Xist colocalizes with Tsix 5’ (33 tsites) (n). A total of n = 625 cells are quantified in this analysis.
Brightline Single Band Filter Sets 166 (Dapi 5060c Nte Zero, Fitc 3540c Nte Zero And Txred 4040c Nte Zero), supplied by IDEX, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/brightline single-band filter sets 166 (dapi-5060c-nte-zero, fitc-3540c-nte-zero and txred-4040c-nte-zero)/product/IDEX
Average 90 stars, based on 1 article reviews
brightline single-band filter sets 166 (dapi-5060c-nte-zero, fitc-3540c-nte-zero and txred-4040c-nte-zero) - by Bioz Stars, 2026-04
90/100 stars
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illumination  (Nikon)
97
Nikon illumination
a Diagram of the Xist/Tsix locus showing Xist exons (green), Tsix exons (magenta), introns (lines), and probe positions for Xist introns (green) and Tsix 3’ introns (magenta). Polymerase positions suggest potential co-transcription of Xist and Tsix. b Conceptual diagram showing expected outcomes with or without TI. If TI occurs, Xist (green) and Tsix 3’ (magenta) signals won’t colocalize (left). Without TI, colocalization occurs (right). c Example image showing <t>DAPI</t> (blue), Xist (green), Tsix 3’ (magenta), and colocalization (white). d-f Similar diagrams and images as (A-C), but for Tsix 5’. With TI, more Xist and Tsix 5’ colocalization occurs than with Tsix 3’ (left); without TI, colocalization remains consistent (right). g-h Joint probability distributions of nascent Tsix (x-axis) and Xist (y-axis) transcripts. Real data is on the left, randomized data on the right. Rho and p values indicate Spearman correlations (real data) and 2D KS test results (randomized data). Analysis includes 625 cells for Tsix 5’ and 651 cells for Tsix 3’. i-n Quantification of colocalization at transcription sites, varying by nascent transcription levels (low, medium, high). Colocalization is plotted by search radius (x-axis, cubic scale) and average events per transcription site (y-axis). Solid line is the mean and shaded area is the standard deviation. Dashed lines is the background fit. Dotted line is the colocalization by chance. i, k, m Quantification of colocalization of Xist with Tsix 3’ depending on the nascent transcription of Xist. Low-level nascent Xist (i), shows colocalization with Tsix 3’ (18 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 3’ (54 tsites) (k), and a lower fraction of high-level Xist colocalizes with Tsix 3’ (11 tsites) (m). A total of n = 651 cells are quantified in this analysis. j, l, n Quantification of colocalization of Xist with Tsix 5’ depending on the nascent transcription of Xist. Low-level nascent Xist (j), shows colocalization with Tsix 5’ (30 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 5’ (68 tsites) (l), and similarly high fraction of high-level Xist colocalizes with Tsix 5’ (33 tsites) (n). A total of n = 625 cells are quantified in this analysis.
Illumination, supplied by Nikon, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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illumination - by Bioz Stars, 2026-04
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tie inverted fluorescence microscope system  (Nikon)
99
Nikon tie inverted fluorescence microscope system
a Diagram of the Xist/Tsix locus showing Xist exons (green), Tsix exons (magenta), introns (lines), and probe positions for Xist introns (green) and Tsix 3’ introns (magenta). Polymerase positions suggest potential co-transcription of Xist and Tsix. b Conceptual diagram showing expected outcomes with or without TI. If TI occurs, Xist (green) and Tsix 3’ (magenta) signals won’t colocalize (left). Without TI, colocalization occurs (right). c Example image showing <t>DAPI</t> (blue), Xist (green), Tsix 3’ (magenta), and colocalization (white). d-f Similar diagrams and images as (A-C), but for Tsix 5’. With TI, more Xist and Tsix 5’ colocalization occurs than with Tsix 3’ (left); without TI, colocalization remains consistent (right). g-h Joint probability distributions of nascent Tsix (x-axis) and Xist (y-axis) transcripts. Real data is on the left, randomized data on the right. Rho and p values indicate Spearman correlations (real data) and 2D KS test results (randomized data). Analysis includes 625 cells for Tsix 5’ and 651 cells for Tsix 3’. i-n Quantification of colocalization at transcription sites, varying by nascent transcription levels (low, medium, high). Colocalization is plotted by search radius (x-axis, cubic scale) and average events per transcription site (y-axis). Solid line is the mean and shaded area is the standard deviation. Dashed lines is the background fit. Dotted line is the colocalization by chance. i, k, m Quantification of colocalization of Xist with Tsix 3’ depending on the nascent transcription of Xist. Low-level nascent Xist (i), shows colocalization with Tsix 3’ (18 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 3’ (54 tsites) (k), and a lower fraction of high-level Xist colocalizes with Tsix 3’ (11 tsites) (m). A total of n = 651 cells are quantified in this analysis. j, l, n Quantification of colocalization of Xist with Tsix 5’ depending on the nascent transcription of Xist. Low-level nascent Xist (j), shows colocalization with Tsix 5’ (30 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 5’ (68 tsites) (l), and similarly high fraction of high-level Xist colocalizes with Tsix 5’ (33 tsites) (n). A total of n = 625 cells are quantified in this analysis.
Tie Inverted Fluorescence Microscope System, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tie inverted fluorescence microscope system/product/Nikon
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tie inverted fluorescence microscope system - by Bioz Stars, 2026-04
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monochrome 12-bit cooled charge-coupled device camera with a maximum resolution of 1,280 × 1,024  (Nikon)
90
Nikon monochrome 12-bit cooled charge-coupled device camera with a maximum resolution of 1,280 × 1,024
a Diagram of the Xist/Tsix locus showing Xist exons (green), Tsix exons (magenta), introns (lines), and probe positions for Xist introns (green) and Tsix 3’ introns (magenta). Polymerase positions suggest potential co-transcription of Xist and Tsix. b Conceptual diagram showing expected outcomes with or without TI. If TI occurs, Xist (green) and Tsix 3’ (magenta) signals won’t colocalize (left). Without TI, colocalization occurs (right). c Example image showing <t>DAPI</t> (blue), Xist (green), Tsix 3’ (magenta), and colocalization (white). d-f Similar diagrams and images as (A-C), but for Tsix 5’. With TI, more Xist and Tsix 5’ colocalization occurs than with Tsix 3’ (left); without TI, colocalization remains consistent (right). g-h Joint probability distributions of nascent Tsix (x-axis) and Xist (y-axis) transcripts. Real data is on the left, randomized data on the right. Rho and p values indicate Spearman correlations (real data) and 2D KS test results (randomized data). Analysis includes 625 cells for Tsix 5’ and 651 cells for Tsix 3’. i-n Quantification of colocalization at transcription sites, varying by nascent transcription levels (low, medium, high). Colocalization is plotted by search radius (x-axis, cubic scale) and average events per transcription site (y-axis). Solid line is the mean and shaded area is the standard deviation. Dashed lines is the background fit. Dotted line is the colocalization by chance. i, k, m Quantification of colocalization of Xist with Tsix 3’ depending on the nascent transcription of Xist. Low-level nascent Xist (i), shows colocalization with Tsix 3’ (18 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 3’ (54 tsites) (k), and a lower fraction of high-level Xist colocalizes with Tsix 3’ (11 tsites) (m). A total of n = 651 cells are quantified in this analysis. j, l, n Quantification of colocalization of Xist with Tsix 5’ depending on the nascent transcription of Xist. Low-level nascent Xist (j), shows colocalization with Tsix 5’ (30 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 5’ (68 tsites) (l), and similarly high fraction of high-level Xist colocalizes with Tsix 5’ (33 tsites) (n). A total of n = 625 cells are quantified in this analysis.
Monochrome 12 Bit Cooled Charge Coupled Device Camera With A Maximum Resolution Of 1,280 × 1,024, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/monochrome 12-bit cooled charge-coupled device camera with a maximum resolution of 1,280 × 1,024/product/Nikon
Average 90 stars, based on 1 article reviews
monochrome 12-bit cooled charge-coupled device camera with a maximum resolution of 1,280 × 1,024 - by Bioz Stars, 2026-04
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inverted fluorescent microscope system  (Nikon)
90
Nikon inverted fluorescent microscope system
a Diagram of the Xist/Tsix locus showing Xist exons (green), Tsix exons (magenta), introns (lines), and probe positions for Xist introns (green) and Tsix 3’ introns (magenta). Polymerase positions suggest potential co-transcription of Xist and Tsix. b Conceptual diagram showing expected outcomes with or without TI. If TI occurs, Xist (green) and Tsix 3’ (magenta) signals won’t colocalize (left). Without TI, colocalization occurs (right). c Example image showing <t>DAPI</t> (blue), Xist (green), Tsix 3’ (magenta), and colocalization (white). d-f Similar diagrams and images as (A-C), but for Tsix 5’. With TI, more Xist and Tsix 5’ colocalization occurs than with Tsix 3’ (left); without TI, colocalization remains consistent (right). g-h Joint probability distributions of nascent Tsix (x-axis) and Xist (y-axis) transcripts. Real data is on the left, randomized data on the right. Rho and p values indicate Spearman correlations (real data) and 2D KS test results (randomized data). Analysis includes 625 cells for Tsix 5’ and 651 cells for Tsix 3’. i-n Quantification of colocalization at transcription sites, varying by nascent transcription levels (low, medium, high). Colocalization is plotted by search radius (x-axis, cubic scale) and average events per transcription site (y-axis). Solid line is the mean and shaded area is the standard deviation. Dashed lines is the background fit. Dotted line is the colocalization by chance. i, k, m Quantification of colocalization of Xist with Tsix 3’ depending on the nascent transcription of Xist. Low-level nascent Xist (i), shows colocalization with Tsix 3’ (18 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 3’ (54 tsites) (k), and a lower fraction of high-level Xist colocalizes with Tsix 3’ (11 tsites) (m). A total of n = 651 cells are quantified in this analysis. j, l, n Quantification of colocalization of Xist with Tsix 5’ depending on the nascent transcription of Xist. Low-level nascent Xist (j), shows colocalization with Tsix 5’ (30 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 5’ (68 tsites) (l), and similarly high fraction of high-level Xist colocalizes with Tsix 5’ (33 tsites) (n). A total of n = 625 cells are quantified in this analysis.
Inverted Fluorescent Microscope System, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
inverted fluorescent microscope system - by Bioz Stars, 2026-04
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Image Search Results


a Diagram of the Xist/Tsix locus showing Xist exons (green), Tsix exons (magenta), introns (lines), and probe positions for Xist introns (green) and Tsix 3’ introns (magenta). Polymerase positions suggest potential co-transcription of Xist and Tsix. b Conceptual diagram showing expected outcomes with or without TI. If TI occurs, Xist (green) and Tsix 3’ (magenta) signals won’t colocalize (left). Without TI, colocalization occurs (right). c Example image showing DAPI (blue), Xist (green), Tsix 3’ (magenta), and colocalization (white). d-f Similar diagrams and images as (A-C), but for Tsix 5’. With TI, more Xist and Tsix 5’ colocalization occurs than with Tsix 3’ (left); without TI, colocalization remains consistent (right). g-h Joint probability distributions of nascent Tsix (x-axis) and Xist (y-axis) transcripts. Real data is on the left, randomized data on the right. Rho and p values indicate Spearman correlations (real data) and 2D KS test results (randomized data). Analysis includes 625 cells for Tsix 5’ and 651 cells for Tsix 3’. i-n Quantification of colocalization at transcription sites, varying by nascent transcription levels (low, medium, high). Colocalization is plotted by search radius (x-axis, cubic scale) and average events per transcription site (y-axis). Solid line is the mean and shaded area is the standard deviation. Dashed lines is the background fit. Dotted line is the colocalization by chance. i, k, m Quantification of colocalization of Xist with Tsix 3’ depending on the nascent transcription of Xist. Low-level nascent Xist (i), shows colocalization with Tsix 3’ (18 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 3’ (54 tsites) (k), and a lower fraction of high-level Xist colocalizes with Tsix 3’ (11 tsites) (m). A total of n = 651 cells are quantified in this analysis. j, l, n Quantification of colocalization of Xist with Tsix 5’ depending on the nascent transcription of Xist. Low-level nascent Xist (j), shows colocalization with Tsix 5’ (30 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 5’ (68 tsites) (l), and similarly high fraction of high-level Xist colocalizes with Tsix 5’ (33 tsites) (n). A total of n = 625 cells are quantified in this analysis.

Journal: bioRxiv

Article Title: Transcriptional stochasticity reveals multiple mechanisms of long non-coding RNA regulation at the Xist – Tsix locus

doi: 10.1101/2025.01.05.631290

Figure Lengend Snippet: a Diagram of the Xist/Tsix locus showing Xist exons (green), Tsix exons (magenta), introns (lines), and probe positions for Xist introns (green) and Tsix 3’ introns (magenta). Polymerase positions suggest potential co-transcription of Xist and Tsix. b Conceptual diagram showing expected outcomes with or without TI. If TI occurs, Xist (green) and Tsix 3’ (magenta) signals won’t colocalize (left). Without TI, colocalization occurs (right). c Example image showing DAPI (blue), Xist (green), Tsix 3’ (magenta), and colocalization (white). d-f Similar diagrams and images as (A-C), but for Tsix 5’. With TI, more Xist and Tsix 5’ colocalization occurs than with Tsix 3’ (left); without TI, colocalization remains consistent (right). g-h Joint probability distributions of nascent Tsix (x-axis) and Xist (y-axis) transcripts. Real data is on the left, randomized data on the right. Rho and p values indicate Spearman correlations (real data) and 2D KS test results (randomized data). Analysis includes 625 cells for Tsix 5’ and 651 cells for Tsix 3’. i-n Quantification of colocalization at transcription sites, varying by nascent transcription levels (low, medium, high). Colocalization is plotted by search radius (x-axis, cubic scale) and average events per transcription site (y-axis). Solid line is the mean and shaded area is the standard deviation. Dashed lines is the background fit. Dotted line is the colocalization by chance. i, k, m Quantification of colocalization of Xist with Tsix 3’ depending on the nascent transcription of Xist. Low-level nascent Xist (i), shows colocalization with Tsix 3’ (18 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 3’ (54 tsites) (k), and a lower fraction of high-level Xist colocalizes with Tsix 3’ (11 tsites) (m). A total of n = 651 cells are quantified in this analysis. j, l, n Quantification of colocalization of Xist with Tsix 5’ depending on the nascent transcription of Xist. Low-level nascent Xist (j), shows colocalization with Tsix 5’ (30 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 5’ (68 tsites) (l), and similarly high fraction of high-level Xist colocalizes with Tsix 5’ (33 tsites) (n). A total of n = 625 cells are quantified in this analysis.

Article Snippet: Fluorescent signal was imaged with Semrock Brightline filters for DAPI (DAPI-5060C-NTE-ZERO), CY5 (NIK-0013 RevA-NTE-ZERO), TAMRA (SpGold-B-NTE-ZERO), and AF488 (FITC-2024B-NTE-ZERO).

Techniques: Standard Deviation